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IF staining and immunohistochemistry For immunohistochemistry staining, the brain was removed and dehydrated in 20% and 30% sucrose solution, then cut into sections at a thickness of 40 m, rinsed in PBS, and incubated in 3% H 2 O 2 to quench endogenous peroxidase activity

In fact, TRPA1 channels are NSCCs that allow Ca 2+ permeation 4

MONITOR Blood glucose every 12 hours Electrolytes every 4 hours Repeat ABG & ketones Monitor vitals, urine output, mental status, ECG Key Goals: Avoid hypoglycemia Gradual glucose reduction Correct acidosis & electrolyte imbalance Clinical Tip: Always monitor potassium closely insulin can cause dangerous shifts

doi: 10.2337/db13-0822

provitamin A carotenoids (e.g., beta-carotene) and preformed vitamin A (e.g., retinol, retinal, retinoic acid, and retinyl esters)

GLP-1 trends in type 2 diabetes Analysis from two EHR (electronic health record) sources in HealthVerity Marketplace shows the rapid adoption of GLP-1 therapy among people with type 2 diabetes